Variation in fluxes estimated from nitrogen isotope discrimination corresponds with independent measures of nitrogen flux in Populus balsamifera L.
Identifieur interne : 001603 ( Main/Exploration ); précédent : 001602; suivant : 001604Variation in fluxes estimated from nitrogen isotope discrimination corresponds with independent measures of nitrogen flux in Populus balsamifera L.
Auteurs : Lee A. Kalcsits [Canada, États-Unis] ; Robert D. Guy [Canada]Source :
- Plant, cell & environment [ 1365-3040 ] ; 2016.
Descripteurs français
- KwdFr :
- Azote (métabolisme), Biomasse (MeSH), Cytoplasme (métabolisme), Développement des plantes (MeSH), Feuilles de plante (métabolisme), Isotopes de l'azote (MeSH), Modèles linéaires (MeSH), Nitrates (métabolisme), Populus (métabolisme), Pousses de plante (métabolisme), Période (MeSH), Racines de plante (métabolisme).
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Nitrates, Nitrogen.
- metabolism : Cytoplasm, Plant Leaves, Plant Roots, Plant Shoots, Populus.
- Biomass, Half-Life, Linear Models, Nitrogen Isotopes, Plant Development.
Abstract
Acquisition of mineral nitrogen by roots from the surrounding environment is often not completely efficient, in which a variable amount of leakage (efflux) relative to gross uptake (influx) occurs. The efflux/influx ratio (E/I) is, therefore, inversely related to the efficiency of nutrient uptake at the root level. Time-integrated estimates of E/I and other nitrogen-use traits may be obtainable from variation in stable isotope ratios or through compartmental analysis of tracer efflux (CATE) using radioactive or stable isotopes. To compare these two methods, Populus balsamifera L. genotypes were selected, a priori, for high or low nitrogen isotope discrimination. Vegetative cuttings were grown hydroponically, and E/I was calculated using an isotope mass balance model (IMB) and compared to E/I calculated using (15) N CATE. Both methods indicated that plants grown with ammonium had greater E/I than nitrate-grown plants. Genotypes with high or low E/I using CATE also had similarly high or low estimates of E/I using IMB, respectively. Genotype-specific means were linearly correlated (r = 0.77; P = 0.0065). Discrepancies in E/I between methods may reflect uncertainties in discrimination factors for the assimilatory enzymes, or temporal differences in uptake patterns. By utilizing genotypes with known variation in nitrogen isotope discrimination, a relationship between nitrogen isotope discrimination and bidirectional nitrogen fluxes at the root level was observed.
DOI: 10.1111/pce.12614
PubMed: 26182898
Affiliations:
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Kalcsits</name><affiliation wicri:level="4"><nlm:affiliation>Department of Forest and Conservation Sciences, University of British Columbia, 2424 Main Mall, Vancouver, BC, Canada, V6T1Z4.</nlm:affiliation><orgName type="university">Université de la Colombie-Britannique</orgName><country>Canada</country><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Horticulture, Washington State University, Tree Fruit Research and Extension Center, 1100 Western Ave. N, Wenatchee, WA, 98801, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Horticulture, Washington State University, Tree Fruit Research and Extension Center, 1100 Western Ave. N, Wenatchee, WA, 98801</wicri:regionArea><wicri:noRegion>98801</wicri:noRegion></affiliation></author><author><name sortKey="Guy, Robert D" sort="Guy, Robert D" uniqKey="Guy R" first="Robert D" last="Guy">Robert D. 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Guy</name><affiliation wicri:level="4"><nlm:affiliation>Department of Forest and Conservation Sciences, University of British Columbia, 2424 Main Mall, Vancouver, BC, Canada, V6T1Z4.</nlm:affiliation><orgName type="university">Université de la Colombie-Britannique</orgName><country>Canada</country><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author></analytic><series><title level="j">Plant, cell & environment</title><idno type="eISSN">1365-3040</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biomass (MeSH)</term><term>Cytoplasm (metabolism)</term><term>Half-Life (MeSH)</term><term>Linear Models (MeSH)</term><term>Nitrates (metabolism)</term><term>Nitrogen (metabolism)</term><term>Nitrogen Isotopes (MeSH)</term><term>Plant Development (MeSH)</term><term>Plant Leaves (metabolism)</term><term>Plant Roots (metabolism)</term><term>Plant Shoots (metabolism)</term><term>Populus (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Azote (métabolisme)</term><term>Biomasse (MeSH)</term><term>Cytoplasme (métabolisme)</term><term>Développement des plantes (MeSH)</term><term>Feuilles de plante (métabolisme)</term><term>Isotopes de l'azote (MeSH)</term><term>Modèles linéaires (MeSH)</term><term>Nitrates (métabolisme)</term><term>Populus (métabolisme)</term><term>Pousses de plante (métabolisme)</term><term>Période (MeSH)</term><term>Racines de plante (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Nitrates</term><term>Nitrogen</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cytoplasm</term><term>Plant Leaves</term><term>Plant Roots</term><term>Plant Shoots</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Azote</term><term>Cytoplasme</term><term>Feuilles de plante</term><term>Nitrates</term><term>Populus</term><term>Pousses de plante</term><term>Racines de plante</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biomass</term><term>Half-Life</term><term>Linear Models</term><term>Nitrogen Isotopes</term><term>Plant Development</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biomasse</term><term>Développement des plantes</term><term>Isotopes de l'azote</term><term>Modèles linéaires</term><term>Période</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Acquisition of mineral nitrogen by roots from the surrounding environment is often not completely efficient, in which a variable amount of leakage (efflux) relative to gross uptake (influx) occurs. The efflux/influx ratio (E/I) is, therefore, inversely related to the efficiency of nutrient uptake at the root level. Time-integrated estimates of E/I and other nitrogen-use traits may be obtainable from variation in stable isotope ratios or through compartmental analysis of tracer efflux (CATE) using radioactive or stable isotopes. To compare these two methods, Populus balsamifera L. genotypes were selected, a priori, for high or low nitrogen isotope discrimination. Vegetative cuttings were grown hydroponically, and E/I was calculated using an isotope mass balance model (IMB) and compared to E/I calculated using (15) N CATE. Both methods indicated that plants grown with ammonium had greater E/I than nitrate-grown plants. Genotypes with high or low E/I using CATE also had similarly high or low estimates of E/I using IMB, respectively. Genotype-specific means were linearly correlated (r = 0.77; P = 0.0065). Discrepancies in E/I between methods may reflect uncertainties in discrimination factors for the assimilatory enzymes, or temporal differences in uptake patterns. By utilizing genotypes with known variation in nitrogen isotope discrimination, a relationship between nitrogen isotope discrimination and bidirectional nitrogen fluxes at the root level was observed.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26182898</PMID><DateCompleted><Year>2016</Year><Month>10</Month><Day>18</Day></DateCompleted><DateRevised><Year>2016</Year><Month>12</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1365-3040</ISSN><JournalIssue CitedMedium="Internet"><Volume>39</Volume><Issue>2</Issue><PubDate><Year>2016</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Plant, cell & environment</Title><ISOAbbreviation>Plant Cell Environ</ISOAbbreviation></Journal><ArticleTitle>Variation in fluxes estimated from nitrogen isotope discrimination corresponds with independent measures of nitrogen flux in Populus balsamifera L.</ArticleTitle><Pagination><MedlinePgn>310-9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/pce.12614</ELocationID><Abstract><AbstractText>Acquisition of mineral nitrogen by roots from the surrounding environment is often not completely efficient, in which a variable amount of leakage (efflux) relative to gross uptake (influx) occurs. The efflux/influx ratio (E/I) is, therefore, inversely related to the efficiency of nutrient uptake at the root level. Time-integrated estimates of E/I and other nitrogen-use traits may be obtainable from variation in stable isotope ratios or through compartmental analysis of tracer efflux (CATE) using radioactive or stable isotopes. To compare these two methods, Populus balsamifera L. genotypes were selected, a priori, for high or low nitrogen isotope discrimination. Vegetative cuttings were grown hydroponically, and E/I was calculated using an isotope mass balance model (IMB) and compared to E/I calculated using (15) N CATE. Both methods indicated that plants grown with ammonium had greater E/I than nitrate-grown plants. Genotypes with high or low E/I using CATE also had similarly high or low estimates of E/I using IMB, respectively. Genotype-specific means were linearly correlated (r = 0.77; P = 0.0065). Discrepancies in E/I between methods may reflect uncertainties in discrimination factors for the assimilatory enzymes, or temporal differences in uptake patterns. By utilizing genotypes with known variation in nitrogen isotope discrimination, a relationship between nitrogen isotope discrimination and bidirectional nitrogen fluxes at the root level was observed.</AbstractText><CopyrightInformation>© 2015 John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kalcsits</LastName><ForeName>Lee A</ForeName><Initials>LA</Initials><AffiliationInfo><Affiliation>Department of Forest and Conservation Sciences, University of British Columbia, 2424 Main Mall, Vancouver, BC, Canada, V6T1Z4.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Horticulture, Washington State University, Tree Fruit Research and Extension Center, 1100 Western Ave. N, Wenatchee, WA, 98801, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guy</LastName><ForeName>Robert D</ForeName><Initials>RD</Initials><AffiliationInfo><Affiliation>Department of Forest and Conservation Sciences, University of British Columbia, 2424 Main Mall, Vancouver, BC, Canada, V6T1Z4.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>10</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Cell Environ</MedlineTA><NlmUniqueID>9309004</NlmUniqueID><ISSNLinking>0140-7791</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009566">Nitrates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009587">Nitrogen Isotopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>N762921K75</RegistryNumber><NameOfSubstance 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Isotopes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D063245" MajorTopicYN="N">Plant Development</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018520" MajorTopicYN="N">Plant Shoots</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">flux analysis</Keyword><Keyword MajorTopicYN="N">isotope discrimination</Keyword><Keyword MajorTopicYN="N">nitrogen</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>01</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>07</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>7</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>7</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>10</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26182898</ArticleId><ArticleId IdType="doi">10.1111/pce.12614</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li><li>États-Unis</li></country><region><li>Colombie-Britannique </li></region><settlement><li>Vancouver</li></settlement><orgName><li>Université de la Colombie-Britannique</li></orgName></list><tree><country name="Canada"><region name="Colombie-Britannique "><name sortKey="Kalcsits, Lee A" sort="Kalcsits, Lee A" uniqKey="Kalcsits L" first="Lee A" last="Kalcsits">Lee A. Kalcsits</name></region><name sortKey="Guy, Robert D" sort="Guy, Robert D" uniqKey="Guy R" first="Robert D" last="Guy">Robert D. Guy</name></country><country name="États-Unis"><noRegion><name sortKey="Kalcsits, Lee A" sort="Kalcsits, Lee A" uniqKey="Kalcsits L" first="Lee A" last="Kalcsits">Lee A. Kalcsits</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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